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ncam1 alexa fluor 488  (R&D Systems)


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    R&D Systems ncam1 alexa fluor 488
    Ncam1 Alexa Fluor 488, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncam1+alexa+fluor+488/Mouse+NCAM-1%2FCD56+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pmc09110333__41467_2022_30416_MOESM19_ESM-20-93-96
    Average 92 stars, based on 1 article reviews
    ncam1 alexa fluor 488 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: .. 3 n atu re research | rep o rtin g su m m ary A p ril2020 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) FACS antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100), EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100), CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-PE-Cy7 (Biolegend 101821, clone M1/69, 1:200), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4, 1:100), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220, 1:50), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. IHC antibodies: Hes1 (Cell signaling technology 11988, 1:200), Yap(D8H1X)XP® (Cell signaling technology 14074S, 1:200), CC10/Scgb1a1 (Santa Cruz sc-9772, 1:50), Syp (Neuromics MO20000), 1:200, CC10/Scgb1a1 (Millipore 07-623, 1:200), RFP/Tomato (Biosource MBS448092, 1:500), CGRP (Sigma-Aldrich C8198, 1:200), BrdU (BD Biosciences 347580, 1:50), Dner (R&D Systems AF2254, 1:50), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313, 1:200), AGER/RAGE (R&D Systems MAB1179, 1:200), Alexa Fluor®488 donkey anti-goat IgG (Invitrogen A-11055, 1:200), Alexa Fluor®488 donkey anti-rabbit IgG (Invitrogen A-21206, 1:200), Alexa Fluor®594 donkey antirabbit IgG (Invitrogen A-21207, 1:200), Alexa Fluor®594 donkey anti-goat IgG (Invitrogen A-11058, 1:200), Alexa Fluor®594 donkey anti-rat IgG (Invitrogen A-21209, 1:200) and Alexa Fluor®488 donkey anti-mouse IgG (Invitrogen A-21202, 1:200).

    Flow Cytometry:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: .. 3 n atu re research | rep o rtin g su m m ary A p ril2020 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) FACS antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100), EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100), CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-PE-Cy7 (Biolegend 101821, clone M1/69, 1:200), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4, 1:100), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220, 1:50), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. IHC antibodies: Hes1 (Cell signaling technology 11988, 1:200), Yap(D8H1X)XP® (Cell signaling technology 14074S, 1:200), CC10/Scgb1a1 (Santa Cruz sc-9772, 1:50), Syp (Neuromics MO20000), 1:200, CC10/Scgb1a1 (Millipore 07-623, 1:200), RFP/Tomato (Biosource MBS448092, 1:500), CGRP (Sigma-Aldrich C8198, 1:200), BrdU (BD Biosciences 347580, 1:50), Dner (R&D Systems AF2254, 1:50), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313, 1:200), AGER/RAGE (R&D Systems MAB1179, 1:200), Alexa Fluor®488 donkey anti-goat IgG (Invitrogen A-11055, 1:200), Alexa Fluor®488 donkey anti-rabbit IgG (Invitrogen A-21206, 1:200), Alexa Fluor®594 donkey antirabbit IgG (Invitrogen A-21207, 1:200), Alexa Fluor®594 donkey anti-goat IgG (Invitrogen A-11058, 1:200), Alexa Fluor®594 donkey anti-rat IgG (Invitrogen A-21209, 1:200) and Alexa Fluor®488 donkey anti-mouse IgG (Invitrogen A-21202, 1:200).

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: Immunoassay antibodies: ASCL1 (Santa Cruz sc-374104, 1:200), YAP1 (D8H1X)XP® (Cell Signaling Technology 14074S, 1:2000), NOTCH2 (Cell Signaling Technology 5732, 1:2000), HES1 (Cell Signaling Technology 11988, 1:50), UCHL1 (Sigma-Aldrich HPA005993, 1:5000), and HSP90 (Cell Signaling Technology 4877, 1:5000). .. The following antibodies were validated for flow cytometry of mouse cells by the manufacturer using respective positive control cells (e.g. mouse splenocytes for CD45) compared to isotype control antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119), CD24-APC (eBioscience 17-0242-82, clone M1/69), F4/80-PE-Cy7 (BioLegend 123114, clone BM8), EpCAM-APC (eBioscience 17-5791-82, clone G8.8), CD45-Pacific Blue (BioLegend 103126, clone 30-F11), CD31-Pacific Blue (BioLegend 102422, clone 390), TER-119-Pacific Blue (BioLegend 116232, clone TER-119), CD24-PE-Cy7 (Biolegend 101821, clone M1/69), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4). .. The following antibodies were validated by manufacturer for IHC /Immunoassay using positive control tissue sections, positive control cell line lysates, gene knockout/knockdown and overexpression experiments: Hes1 (Cell signaling technology 11988), Yap(D8H1X)XP® (Cell signaling technology 14074S), CC10/Scgb1a1 (Santa Cruz sc-9772), Syp (Neuromics MO20000), CC10/Scgb1a1 (Millipore 07-623), RFP/Tomato (Biosource MBS448092), CGRP (Sigma-Aldrich C8198), BrdU (BD Biosciences 347580), Dner (R&D Systems AF2254), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313), AGER/RAGE (R&D Systems MAB1179), ASCL1 (Santa Cruz sc-374104), NOTCH2 (Cell Signaling Technology 5732), UCHL1 (Sigma-Aldrich HPA005993), and HSP90 (Cell Signaling Technology 4877).

    Magnetic Resonance Imaging:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: .. 3 n atu re research | rep o rtin g su m m ary A p ril2020 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) FACS antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100), EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100), CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-PE-Cy7 (Biolegend 101821, clone M1/69, 1:200), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4, 1:100), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220, 1:50), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. IHC antibodies: Hes1 (Cell signaling technology 11988, 1:200), Yap(D8H1X)XP® (Cell signaling technology 14074S, 1:200), CC10/Scgb1a1 (Santa Cruz sc-9772, 1:50), Syp (Neuromics MO20000), 1:200, CC10/Scgb1a1 (Millipore 07-623, 1:200), RFP/Tomato (Biosource MBS448092, 1:500), CGRP (Sigma-Aldrich C8198, 1:200), BrdU (BD Biosciences 347580, 1:50), Dner (R&D Systems AF2254, 1:50), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313, 1:200), AGER/RAGE (R&D Systems MAB1179, 1:200), Alexa Fluor®488 donkey anti-goat IgG (Invitrogen A-11055, 1:200), Alexa Fluor®488 donkey anti-rabbit IgG (Invitrogen A-21206, 1:200), Alexa Fluor®594 donkey antirabbit IgG (Invitrogen A-21207, 1:200), Alexa Fluor®594 donkey anti-goat IgG (Invitrogen A-11058, 1:200), Alexa Fluor®594 donkey anti-rat IgG (Invitrogen A-21209, 1:200) and Alexa Fluor®488 donkey anti-mouse IgG (Invitrogen A-21202, 1:200).

    Biomarker Discovery:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: .. 3 n atu re research | rep o rtin g su m m ary A p ril2020 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) FACS antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100), EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100), CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-PE-Cy7 (Biolegend 101821, clone M1/69, 1:200), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4, 1:100), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220, 1:50), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. IHC antibodies: Hes1 (Cell signaling technology 11988, 1:200), Yap(D8H1X)XP® (Cell signaling technology 14074S, 1:200), CC10/Scgb1a1 (Santa Cruz sc-9772, 1:50), Syp (Neuromics MO20000), 1:200, CC10/Scgb1a1 (Millipore 07-623, 1:200), RFP/Tomato (Biosource MBS448092, 1:500), CGRP (Sigma-Aldrich C8198, 1:200), BrdU (BD Biosciences 347580, 1:50), Dner (R&D Systems AF2254, 1:50), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313, 1:200), AGER/RAGE (R&D Systems MAB1179, 1:200), Alexa Fluor®488 donkey anti-goat IgG (Invitrogen A-11055, 1:200), Alexa Fluor®488 donkey anti-rabbit IgG (Invitrogen A-21206, 1:200), Alexa Fluor®594 donkey antirabbit IgG (Invitrogen A-21207, 1:200), Alexa Fluor®594 donkey anti-goat IgG (Invitrogen A-11058, 1:200), Alexa Fluor®594 donkey anti-rat IgG (Invitrogen A-21209, 1:200) and Alexa Fluor®488 donkey anti-mouse IgG (Invitrogen A-21202, 1:200).

    FACS:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: .. 3 n atu re research | rep o rtin g su m m ary A p ril2020 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Human research participants Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines Cell line source(s) FACS antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11, 1:100), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390, 1:100), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100), EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100), CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-PE-Cy7 (Biolegend 101821, clone M1/69, 1:200), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4, 1:100), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220, 1:50), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. IHC antibodies: Hes1 (Cell signaling technology 11988, 1:200), Yap(D8H1X)XP® (Cell signaling technology 14074S, 1:200), CC10/Scgb1a1 (Santa Cruz sc-9772, 1:50), Syp (Neuromics MO20000), 1:200, CC10/Scgb1a1 (Millipore 07-623, 1:200), RFP/Tomato (Biosource MBS448092, 1:500), CGRP (Sigma-Aldrich C8198, 1:200), BrdU (BD Biosciences 347580, 1:50), Dner (R&D Systems AF2254, 1:50), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313, 1:200), AGER/RAGE (R&D Systems MAB1179, 1:200), Alexa Fluor®488 donkey anti-goat IgG (Invitrogen A-11055, 1:200), Alexa Fluor®488 donkey anti-rabbit IgG (Invitrogen A-21206, 1:200), Alexa Fluor®594 donkey antirabbit IgG (Invitrogen A-21207, 1:200), Alexa Fluor®594 donkey anti-goat IgG (Invitrogen A-11058, 1:200), Alexa Fluor®594 donkey anti-rat IgG (Invitrogen A-21209, 1:200) and Alexa Fluor®488 donkey anti-mouse IgG (Invitrogen A-21202, 1:200).

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: Lungs from Chga-GFP mice were digested and stained as above with an additional lineage negative selection marker F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100) and EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100) instead of CD24 as positive selection marker. .. Tumors isolated from the lungs of TKO;Rest fl/fl or TKO;Yap1 fl/fl mice were digested in the same way but stained with the following FACS antibodies: CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50), and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. FACS was performed with a 100 μm nozzle on a BD FACSAria II using the FACSDiva software.

    Isolation:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: Lungs from Chga-GFP mice were digested and stained as above with an additional lineage negative selection marker F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100) and EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100) instead of CD24 as positive selection marker. .. Tumors isolated from the lungs of TKO;Rest fl/fl or TKO;Yap1 fl/fl mice were digested in the same way but stained with the following FACS antibodies: CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50), and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. FACS was performed with a 100 μm nozzle on a BD FACSAria II using the FACSDiva software.

    Staining:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: Lungs from Chga-GFP mice were digested and stained as above with an additional lineage negative selection marker F4/80-PE-Cy7 (BioLegend 123114, clone BM8, 1:100) and EpCAM-APC (eBioscience 17-5791-82, clone G8.8, 1:100) instead of CD24 as positive selection marker. .. Tumors isolated from the lungs of TKO;Rest fl/fl or TKO;Yap1 fl/fl mice were digested in the same way but stained with the following FACS antibodies: CD45-Pacific Blue (BioLegend 103126, clone 30-F11, 1:100), CD31-Pacific Blue (BioLegend 102422, clone 390, 1:100), TER-119-Pacific Blue (BioLegend 116232, clone TER-119, 1:100), CD24-APC (eBioscience 17-0242-82, clone M1/69, 1:200), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220, 1:50), and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4, 1:100). .. FACS was performed with a 100 μm nozzle on a BD FACSAria II using the FACSDiva software.

    Positive Control:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: Immunoassay antibodies: ASCL1 (Santa Cruz sc-374104, 1:200), YAP1 (D8H1X)XP® (Cell Signaling Technology 14074S, 1:2000), NOTCH2 (Cell Signaling Technology 5732, 1:2000), HES1 (Cell Signaling Technology 11988, 1:50), UCHL1 (Sigma-Aldrich HPA005993, 1:5000), and HSP90 (Cell Signaling Technology 4877, 1:5000). .. The following antibodies were validated for flow cytometry of mouse cells by the manufacturer using respective positive control cells (e.g. mouse splenocytes for CD45) compared to isotype control antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119), CD24-APC (eBioscience 17-0242-82, clone M1/69), F4/80-PE-Cy7 (BioLegend 123114, clone BM8), EpCAM-APC (eBioscience 17-5791-82, clone G8.8), CD45-Pacific Blue (BioLegend 103126, clone 30-F11), CD31-Pacific Blue (BioLegend 102422, clone 390), TER-119-Pacific Blue (BioLegend 116232, clone TER-119), CD24-PE-Cy7 (Biolegend 101821, clone M1/69), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4). .. The following antibodies were validated by manufacturer for IHC /Immunoassay using positive control tissue sections, positive control cell line lysates, gene knockout/knockdown and overexpression experiments: Hes1 (Cell signaling technology 11988), Yap(D8H1X)XP® (Cell signaling technology 14074S), CC10/Scgb1a1 (Santa Cruz sc-9772), Syp (Neuromics MO20000), CC10/Scgb1a1 (Millipore 07-623), RFP/Tomato (Biosource MBS448092), CGRP (Sigma-Aldrich C8198), BrdU (BD Biosciences 347580), Dner (R&D Systems AF2254), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313), AGER/RAGE (R&D Systems MAB1179), ASCL1 (Santa Cruz sc-374104), NOTCH2 (Cell Signaling Technology 5732), UCHL1 (Sigma-Aldrich HPA005993), and HSP90 (Cell Signaling Technology 4877).

    Control:

    Article Title: A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Article Snippet: Immunoassay antibodies: ASCL1 (Santa Cruz sc-374104, 1:200), YAP1 (D8H1X)XP® (Cell Signaling Technology 14074S, 1:2000), NOTCH2 (Cell Signaling Technology 5732, 1:2000), HES1 (Cell Signaling Technology 11988, 1:50), UCHL1 (Sigma-Aldrich HPA005993, 1:5000), and HSP90 (Cell Signaling Technology 4877, 1:5000). .. The following antibodies were validated for flow cytometry of mouse cells by the manufacturer using respective positive control cells (e.g. mouse splenocytes for CD45) compared to isotype control antibodies: CD45-PE-Cy7 (eBioscience 25-0451-82, clone 30-F11), CD31-PE-Cy7 (eBioscience 25-0311-82, clone 390), TER-119-PE-Cy7 (eBioscience 25-5921-82, clone TER-119), CD24-APC (eBioscience 17-0242-82, clone M1/69), F4/80-PE-Cy7 (BioLegend 123114, clone BM8), EpCAM-APC (eBioscience 17-5791-82, clone G8.8), CD45-Pacific Blue (BioLegend 103126, clone 30-F11), CD31-Pacific Blue (BioLegend 102422, clone 390), TER-119-Pacific Blue (BioLegend 116232, clone TER-119), CD24-PE-Cy7 (Biolegend 101821, clone M1/69), ICAM1/CD54-PerCP-Cy5.5 (Biolegend 116123, clone YN1/1.7.4), NCAM1/CD56-APC (R&D Systems FAB7820A, clone 809220), NCAM1-Alexa Fluor 488 (R&D Systems FAB7820G, clone 809220) and ICAM1-PE-CY7 (BioLegend 116122, clone YN1/1.7.4). .. The following antibodies were validated by manufacturer for IHC /Immunoassay using positive control tissue sections, positive control cell line lysates, gene knockout/knockdown and overexpression experiments: Hes1 (Cell signaling technology 11988), Yap(D8H1X)XP® (Cell signaling technology 14074S), CC10/Scgb1a1 (Santa Cruz sc-9772), Syp (Neuromics MO20000), CC10/Scgb1a1 (Millipore 07-623), RFP/Tomato (Biosource MBS448092), CGRP (Sigma-Aldrich C8198), BrdU (BD Biosciences 347580), Dner (R&D Systems AF2254), RBP-J/RBPSUH (D10A4)XP® (Cell Signaling Technology 5313), AGER/RAGE (R&D Systems MAB1179), ASCL1 (Santa Cruz sc-374104), NOTCH2 (Cell Signaling Technology 5732), UCHL1 (Sigma-Aldrich HPA005993), and HSP90 (Cell Signaling Technology 4877).



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    Average 92 stars, based on 1 article reviews
    alexa fluor 488 goat anti rabbit igg - by Bioz Stars, 2026-10
    92/100 stars
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